1. Production and cryopreservation of definitive endoderm from human pluripotent stem cells under defined and scalable culture conditions. Sahabian A, Dahlmann J, Martin U, Olmer R. 2021 Nature Protocols 16, 1581–1599.

2. Pre-clinical optimisation and safety studies of a new lentiviral gene therapy for p47phox -deficient chronic granulomatous disease. Schejtman A, Vetharoy W, Choi U, Rivat C, Theobald N, Piras G, Leon-Rico D, Buckland K, Armenteros-Monterroso E, Benedetti S, Ashworth MT, Rothe M, Schambach A, Gaspar HB, Kang E, Malech HL, Thrasher AJ, Santilli G. Hum Gene Ther. 2021 Mar 19. doi: 10.1089/hum.2020.276.

3. Streptococcus suis Induces Expression of Cyclooxygenase-2 in Porcine Lung Tissue. Dresen M, Schenk J, Weldearegay JB, Vötsch D, Baumgärtner W, Valentin-Weigand P, Nerlich A. 2021 Microorganisms, 9,366. doi:10.1101/2020.11.10.371575.

4. Animal models for cystic fibrosis: a systematic search and mapping review of the literature. Part 2: nongenetic models. Cathalijn HC Leenaars, Rob BM de Vries, Joey Reijmer, David Holthaus, Damian Visser, Anna Heming, Janneke Elzinga, Rosalie WM Kempkes, Wouter Beumer, Carine Punt, Franck LB Meijboom, Merel Ritskes-Hoitinga. 2021 Laboratory animals (10.1177/0023677221990688).

5. Risk-Based Decision Making: A Systematic Scoping Review of Animal Models and a Pilot Study on the Effects of Sleep Deprivation in Rats. Cathalijn H.C. Leenaars, Stevie Van der Mierden, Ruud N.J.M.A. Joosten, Marnix A. Van der Weide, Mischa Schirris, Maurice Dematteis, Franck L.B. Meijboom, Matthijs G.P. Feenstra and André Bleich. 2021 Clocks & sleep (10.3390/clockssleep3010003).

6. Extracting data from graphs: A case‐study on animal research with implications for meta‐analyses. Stevie Van der Mierden Loukia Maria Spineli Steven R. Talbot Christina Yiannakou Eva Zentrich Nora Weegh Birgitta Struve Talke Friederike Zur Brügge André Bleich Cathalijn H.C. Leenaars. 2021 Research synthesis methods (10.1002/jrsm.14 81).

7. R2N and the Use of Alternative Methods in COVID-19 Research. Boyle E., Bleich A., 2020 ALTEX. 37(4), doi:10.14573/altex.2010011.

8. Neutrophils exhibit an individual response to different oral bacterial biofilms. Mikolai C, Branitzki-Heinemann K, Ingendoh-Tsakmakidis A, Stiesch M, von Köckritz-Blickwede M, Winkel A., Journal of Oral Microbiology 2020 Dez;13(1):1856565. DOI: 10.1080/20002297.2020.1856565.

9. Bordetella bronchiseptica promotes adherence, colonization, and cytotoxicity of Streptococcus suis in a porcine precision-cut lung slice model. Vötsch D, Willenborg M, Baumgärtner W, Rohde M, Valentin-Weigand P., 2020 Virulence, 12:1,84-95, doi: 10.1080/21505594.2020.1858604.

10. Multiple Genes Surrounding Bcl-x L, a Common Retroviral Insertion Site, Can Influence Hematopoiesis Individually or in Concert. Ha TC, Stahlhut M, Rothe M, Paul G, Dziadek V, Morgan M, Brugman M, Fehse B, Kustikova O, Schambach A, Baum C., Hum Gene Ther 2020 Dec 7. doi: 10.1089/hum.2019.344.

11. A Systematic Review Comparing Experimental Design of Animal and Human Methotrexate Efficacy Studies for Rheumatoid Arthritis: Lessons for the Translational Value of Animal Studies. Leenaars, C., Stafleu, F., de Jong, D., van Berlo, M., Geurts, T., Coenen-de Roo, T., Prins, J. B., Kempkes, R., Elzinga, J., Bleich, A., de Vries, R., Meijboom, F., and Ritskes-Hoitinga, M., Animals 2020; 10:6. doi: 10.3390/ani10061047.

12. Integrating Philosophy of Science Into Research on Ethical, Legal and Social Issues in the Life Science. Lohse, S., Wasmer, M. & Reydon, T. 2020 Perspectives on Science 28(6): 700-736.

13. Reviewing the animal literature: how to describe and choose between different types of literature reviews. Leenaars, C., Tsaioun, K., Stafleu, F., Rooney, K., Meijboom, F., Ritskes-Hoitinga, M., and Bleich, A. 2020. Lab Anim 2020: 23677220968599. doi: 10.1177/0023677220968599.

14. Measuring endogenous corticosterone in laboratory mice – a mapping review, meta-analysis, and open source database. Van der Mierden, S., Leenaars, C. H. C., Boyle, E. C., Ripoli, F. L., Gass, P., Durst, M., Goerlich-Jansson, V. C., Jirkof, P., Keubler, L. M., Talbot, S. R., Habedank, A., Lewejohann, L., Tolba, R. H., and Bleich, A., ALTEX, 2020. doi: 10.14573/altex.2004221.

15. Farm animal-derived models of the intestinal epithelium: Recent advances and future applications of intestinal organoids. Seeger, B., ATLA 2020, DOI: 10.1177/0261192920974026.

16. Neutrophils exhibit an individual response to different oral bacterial biofilms. Mikolai C, Branitzki-Heinemann K, Ingendoh-Tsakmakidis A, Stiesch M, von Köckritz-Blickwede M, Winkel A., Journal of Oral Microbiology 2020 Dez;13(1):1856565. DOI: 10.1080/20002297.2020.1856565.

17. Early host-microbe interaction in a peri-implant oral mucosa-biofilm model. Mikolai C, Kommerein N, Ingendoh-Tsakmakidis A, Winkel A, Falk CS, Stiesch M., Cell Microbiol. 2020 Aug;22(8):e13209. doi: 10.1111/cmi.13209. Epub 2020 May 14.

18. CD14 and ALPK1 Affect Expression of Tight Junction Components and Proinflammatory Mediators upon Bacterial Stimulation in a Colonic 3D Organoid Model. Pascal Brooks/Talke zur Bruegge, Erin C. Boyle, Stefan Kalies, Santiago Nahuel Villarreal, Andrea Liese, André Bleich, and Manuela Buettner. Stem Cells International, vol. 2020, Article ID 4069354, 11 pages, 2020. doi: 10.1155/2020/4069354

20. Influence of Oxygen on Function and Cholesterol Composition of Murine Bone Marrow-Derived Neutrophils. Branitzki-Heinemann K, Brogden G, von Köckritz-Blickwede M., Methods Mol Biol. 2020;2087:223-233. doi: 10.1007/978-1-0716-0154-9_17.

21. R2N Science Camp. van der Mierden S, Leenaars CHC, Bleich A, 2020 ALTEX. 37(2), doi:10.14573/altex.2002211.

22. Effects of commensal biofilms on a peri-implant mucosa in a three dimensional model. Mikolai C, Ingendoh-Tsakmakidis A, Winkel A, Kommerein N, Falk CS, Rossi A, Walles H, Stiesch M., Biomed Tech (Berl). 2019 Feb 1;64(s1):63-65. doi: 10.1515/bmt-2019-7010.

23. Commensal and pathogenic biofilms differently modulate peri-implant oral mucosa in an organotypic model. Ingendoh-Tsakmakidis A, Mikolai C, Winkel A, Szafrański SP, Falk CS, Rossi A, Walles H, Stiesch M., Cell Microbiol. 2019 Oct;21(10):e13078. doi: 10.1111/cmi.13078. Epub 2019 Jul 17.

24. Generation of a NKX2.1 – p63 double transgenic knock-in reporter cell line from human induced pluripotent stem cells (MHHi006-A-4). Drick N, Sahabian A, Pongpamorn P, Merkert S, Göhring G, Welte T, Martin U, Olmer R., Stem Cell Res. 2019 Nov 19;42:101659.

25. Generation of a NKX2.1 knock-in reporter cell line from human induced pluripotent stem cells (MHHi006-A-2). Olmer R, Dahlmann J, Merkert S, Baus S, Göhring G, Martin U., Stem Cell Res. 2019 Aug;39:101492.

26. Chemically-Defined, Xeno-Free, Scalable Production of hPSC-Derived Definitive Endoderm Aggregates with Multi-Lineage Differentiation Potential. Sahabian A, Sgodda M, Naujok O, Dettmer R, Dahlmann J, Manstein F, Cantz T, Zweigerdt R, Martin U, Olmer R., Cells. 2019 Dec 4;8(12).

27. How Ethics, Law and Philosophy of Science Can Help Make Progress in the Development and Use of Alternative Methods. Hermann, L., Hoppe, N., Kahrass H., Lohse, S., Mertz, M. & Pietschmann, I., 2019 ALTEX – Alternatives to Animal Experimentation 36(4): 681.

28. Sleep and Microdialysis: An experiment and a Systematized Review of Histamine and Several Amino Acids. Leenaars CH, Drinkenburg WH, Nolten C, Dematteis M, Joosten RN, Feenstra MG, De Vries RB. J Circadian Rhythms 2019; 17:7. doi: 10.5334/jcr.183.

29. Software tools for literature screening in systematic reviews in biomedical research. Van der Mierden, S., Tsaioun, K., Bleich, A., and Leenaars, C. H. C., ALTEX 2019; 36:3. doi: 10.14573/altex.1902131.

30. Animal to human translation: a systematic scoping review of reported concordance rates. Leenaars CH, Kouwenaar C, Stafleu FR, Bleich A, Ritskes-Hoitinga M, De Vries RB, Meijboom FL., J Transl Med. 2019;17(1):223. doi: 10.1186/s12967-019-1976-2.

31. Animal models for cystic fibrosis: A systematic search and mapping review of the literature – Part 1: genetic models. Leenaars CH, De Vries RB, Heming A, Visser D, Holthaus D, Reijmer J, Elzinga J, Kempkes RW, Punt C, Beumer W, Meijboom FL, Ritskes-Hoitinga M., Lab Anim. 2019:23677219868502. doi: 10.1177/0023677219868502.

32. Measurement of corticosterone in mice: a protocol for a mapping review. Leenaars CH, van der Mierden S, Durst M, Goerlich-Jansson VC, Ripoli FL, Keubler LM, Talbot SR, Boyle E, Habedank A, Jirkof P, Lewejohann L., Laboratory animals. 2019 Oct 26:0023677219868499.

33. Brain Microdialysate Monoamines in Relation to Circadian Rhythms, Sleep, and Sleep Deprivation – a Systematic Review, Network Meta-analysis, and New Primary Data. Menon, JML, Nolten, C, Achterberg, EJM, Joosten, RNJMA, Dematteis, M, Feenstra, MGP, Drinkenburg, WH(Pim) and Leenaars, CHC. 2019 Journal of Circadian Rhythms, 17(1): 1, pp. 1–32. DOI:

34. Alternative methods to replace or reduce animal models in biomedical research. Lüder Ripoli F, Buettner M, Bleich A, 2019 ALTEX. 36(1), doi:10.14573/altex.1812171.

35. An evolutionary divergent pestivirus lacking the N pro gene systemically infects a whale species. Wendy K Jo, Cornelis van Elk, Marco van de Bildt, Peter van Run, Monique Petry, Sonja T Jesse, Klaus Jung, Martin Ludlow, Thijs Kuiken, Albert Osterhaus. 2019 Emerging Microbes and Infections.

36. A Systematic Search and Mapping Review of Studies on Intracerebral Microdialysis of Amino Acids, and Systematized Review of Studies on Circadian Rhythms. Leenaars, CHC, Freymann, J, Jakobs, K, Menon, JML, Van Ee, TJ, Elzinga, J, Kempkes, RVM, Zoer, B and Drinkenburg, PWHIM. 2018 Journal of Circadian Rhythms, 16(1): 12, pp. 1–11. DOI:

37. Intracerebral Adenosine During Sleep Deprivation: A Meta-Analysis and New Experimental Data. Leenaars, CHC, Savelyev, SA, Van der Mierden, S, Joosten, RNJMA, Dematteis, M, Porkka-Heiskanen, Tand Feenstra, MGP. 2018 Journal of Circadian Rhythms, 16(1): 11, pp. 1–11. DOI:

38. Intracerebral microdialysis of adenosine and adenosine monophosphate – a systematic review and meta-regression analysis of baseline concentrations. van der Mierden S, Savelyev SA, IntHout J, de Vries RBM, Leenaars CHC. J Neurochem. 2018;147(1):58-70. doi: 10.1111/jnc.14552.

39. Evolutionary evidence for multi-host transmission of cetacean morbillivirus. Wendy K Jo, Jochen Kruppa, Andre Habierski, Marco van de Bildt, Sandro Mazzariol, Giovanni Di Guardo, Ursula Siebert, Thijs Kuiken, Klaus Jung, Albert Osterhaus, Martin Ludlow. 2018 Emerging Microbes and Infections.

40. The Legal Governance of Animal Biotechnologies. Hoppe, Nils. 2018 Aninmal Biotechnology 2. Emerging breeding technologies. Berlin: Springer.

41. CRISPR/Cas9 Immunoengineering of Hoxb8-Immortalized Progenitor Cells for Revealing CCR7-Mediated Dendritic Cell Signaling and Migration Mechanisms in vivo. Hammerschmidt SI, Werth K, Rothe M, Galla M, Permanyer M,Patzer GE, Bubke A, Frenk DN, Selich A, Lange L, Schambach A, Bošnjak B, Förster R., Front Immunol 2018; 9: 1949.

42. Pre-clinical Development of a Lentiviral Vector Expressing the Anti-sickling βAS3 Globin for Gene Therapy for Sickle Cell Disease. Poletti V, Urbinati F, Charrier S, Corre G,Hollis RP, Campo Fernandez B, Martin S, Rothe M, Schambach A, Kohn DB, Mavilio FB., Mol Ther Methods Clin Dev. 2018 Dec 14; 11: 167–179.

43. Formation of Neutrophil Extracellular Traps under Low Oxygen Level. Branitzki-Heinemann K, Möllerherm H, Völlger L, Husein DM, de Buhr N, Blodkamp S, Reuner F, Brogden G, Naim HY, von Köckritz-Blickwede M, Front. Immunol. 8, 541 (2017).

44. Hypoxia Modulates the Response of Mast Cells to Staphylococcus aureus Infection. Front. Möllerherm H, Branitzki-Heinemann K, Brogden G, Elamin AA, Oehlmann W, Fuhrmann H, Singh M, Naim HY, von Köckritz-Blickwede M, Immunol. 8, 541 (2017).

45. A Scalable Approach for the Generation of Human Pluripotent Stem Cell-Derived Hepatic Organoids with Sensitive Hepatotoxicity Features. Sgodda, M., Dai, Z., Zweigerdt, R., Sharma, A. D., Ott, M., and Cantz, T., Stem Cells Dev 26(20):1490.